The main aim of the proposed project is to optimize the unconventional methods of hybridization via protoplast fusion techniques in economically important cultivated crops of the genus Brassica, Cucumis and Solanum. The mothods of protoplasttechnology should help in developing the specific gene combination in studied plant species, which are impossible to obtain through the conventional breeding approaches. Further this techniques offer a convenient way for overcoming the limitations on sexual hybridization in plant breeding. The goal of protoplasts fusion is to control the transfer of an agriculturally important traits, such as disease resistance, abiotic stresses and also the improvement of the quality characteristics of seeds.
The proposed investigations of this research are focussed on the development of the methods for symmetrical and asymmetrical protoplast fusion. The fusions are supposed to be induced through both electroporation (electrical stimulation) as well as chemical fusion of protoplasts in cultivated and wild species of the genus Brassica, Cucumis and Solanum. The studied genotypes will be evaluatedon the basis of the following parameters: protoplast culture efficiency, protoplast viability and regeneration of protoplasts. The step-by- step
Process of somatic hybridization will be controlled and analysed employing the cytological methods. The extent of obtained hybrid products from fusions and regenerants will be evaluated by means of cytological as well as molecular biology methods. Developed plant materials should help in broadening the genetical variability in plant species under investigation
List of main research projects of the Department of Botany
RESEARCH PROJECTS